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Welcome to the pKD server! This server lets you pick a PDB file, specify how much and in which direction you want to change a pKa value of a titratable group in this protein, and subsequently the server calculates a set of point mutations that will cause the desired pKa change. The algorithm and the results that it generates are described in Tynan-Connolly, B.M. & Nielsen J.E. Re-Designing protein pKa values Protein science 2007;16 239-249, but if you have any questions that are not covered in the paper then feel free to write Jens.Nielsen@ucd.ie Select protein --> --> |
| <type 'exceptions.OSError'> | Python 2.7.1+: /usr/bin/python Sat Jan 28 13:23:39 2012 |
A problem occurred in a Python script. Here is the sequence of function calls leading up to the error, in the order they occurred.
| /var/www/cgi-bin/pKa_Design/server_start.cgi in |
| 64 return |
| 65 |
| 66 |
| 67 if __name__=="__main__": |
| => 68 main() |
| main = <function main> |
| /var/www/cgi-bin/pKa_Design/server_start.cgi in main() |
| 34 #print text |
| 35 import pKaDesign_server |
| => 36 text2,preps=pKaDesign_server.find_prepared_proteins() |
| 37 text=text+text2 |
| 38 print text |
| text2 undefined, preps undefined, pKaDesign_server = <module 'pKaDesign_server' from '/var/www/cgi-bin/pKa_Design/pKaDesign_server.py'>, pKaDesign_server.find_prepared_proteins = <function find_prepared_proteins> |
| /var/www/cgi-bin/pKa_Design/pKaDesign_server.py in find_prepared_proteins(client=None) |
| 57 and then looking for the tabdata_complete file""" |
| 58 text='' |
| => 59 dirs=os.listdir(datadir) |
| 60 prepared_proteins=[] |
| 61 pkacalc_info=[] |
| dirs undefined, global os = <module 'os' from '/usr/lib/python2.7/os.pyc'>, os.listdir = <built-in function listdir>, global datadir = '/home/pKa_Design/data' |
<type 'exceptions.OSError'>: [Errno 2] No such file or directory: '/home/pKa_Design/data'
args =
(2, 'No such file or directory')
errno =
2
filename =
'/home/pKa_Design/data'
message =
''
strerror =
'No such file or directory'